Volume 23

Preliminary research for developing the new method of the calibration of microplate readers in GUM – comparative analysis of the results

Authors Dobrochna Matkowska, Iwona Ostrowska, Jolanta Gębicka, Łukasz Litwiniuk - Central Office of Measures (Główny Urząd Miar)

Abstract

In the coming years, it is planned to develop measurement methods and measurement stand for the calibration of microplate readers and control standards thereof i.e. to create mechanisms linking the spectral transmittance with reference to the primary measurement standard (Cary 5000 spectrophotometer) with a group of modern spectrophotometric devices – microplate readers.

Bibliography

[1] T. Nowicka-Jankowska, E. Wieteska, K. Gorczyńska, A. Michalik: Spektrofotometria UV-VIS w analizie chemicznej. PWN 1988.
[2] J. Gębicka, A. Rębecka, A. Żórawski: Wzorcowanie Spektrofotometrów – Źródła Błędów (Cz. 1). Prace Instytutu Elektrotechniki, zeszyt 237, 2008.
[3] G. Weiland: The enzyme-linked immunosorbent assay (ELISA)--a new serodiagnostic method for the detection of parasitic infections (author’s transl). MMW 1978.
[4] Materiał wytworzony przez Zakład Optyki na użytek GUM.
[5] https://www.starna.com/uv-absorbance
[6] https://www.agilent.com/en/products/uv-vis-uv-vis-nir/ uv-vis-uv-vis-nir-systems/cary-5000-uv-vis-nir

microplate readers, primary standard, spectral transmittance

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